Expression and Purification of Thioredoxin 2 and Thioredoxin 3 from Streptomyces coelicolor A3 (2)

Authors

  • M. Koháryová Department of Biochemistry, Faculty of Natural Science, Comenius University, Bratislava
  • I. Barák Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava
  • M. Kollárová Department of Biochemistry, Faculty of Natural Science, Comenius University, Bratislava

Keywords:

thioredoxin system, thioredoxin

Abstract

The thioredoxin system is a significant redox regulator in all organisms. Thioredoxins in bacteria are the major dithiol reductants in the cytosol (or an advanced equivalent to dithiotreitol of cells) thanks to the low redox potentials (Holmgren, 1985). In the genome of the studied model Streptomyces coelicolor A3(2) several genes were revealed which code proteins forming the thioredoxin system. It seems that this gram-positive soil bacteria have a very complex redox system, with a variety of reducing possibi­lities. In this work cloning, purification and characterization of further thioredoxins (TrxA2 and TrxA3) are described. Both proteins were overexpressed in E. coli cytoplasma as soluble active hexahistidine fusion proteins and isolated as homogenous substances.

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Published

2012-05-15

How to Cite

Koháryová, M., Barák, I., & Kollárová, M. (2012). Expression and Purification of Thioredoxin 2 and Thioredoxin 3 from Streptomyces coelicolor A3 (2). Chemické Listy, 106(5), 398–403. Retrieved from http://www-.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/946

Issue

Vol. 106 No. 5 (2012)

Section

Articles

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